Apply the first primary antibody, followed by the appropriate secondary antibody and chromogen (recommended to use the most Digest or retrieve the tissue and block for endogenous peroxidase and non-specific To use, simply deparaffinize tissue and hydrate to water. Get Started: How to Implement Denaturing Solution in Your Protocol no retrieval, enzyme digestion, high pH or low pH retrieval solutions). On the antigen retrieval method used (i.e. Solution “serves to remove previously bound primary and link antibodies leaving only the deposit of chromogen from the previous steps, thusĮliminating any potential for cross-reactivity.”2 The solution is supplied as 2 separate components that are mixed at different dilutions depending In order to limit cross-reactivity, an elution step is required.īiocare’s Denaturing Solution can be used as an elution step when performing a sequential double stain and/or multiplex technique. Makes a direct labeling approach impractical. Infrequently, the best-suited antibodies for a study are only available as the same IgG isotype of the same species, and the scarcity of the antigen ![]() Species or of different IgG isotypes to ensure the specific labeling with the secondary detection systems.1 With an abundance of mouse primaryĪntibodies and increase of rabbit primary antibodies, it is easier than ever to conduct chromogenic multiplex (IHC) staining. A Rev-elution-ary Solution to Eliminate Cross-Reactivity! How to Utilize a Denaturing Solution Step to Eliminate Cross-Reactivityĭouble or multiple antigen labeling in immunohistochemistry (IHC) classically relies on the existence of primary antibodies raised in different
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